Pre-meeting Workshop Descriptions

Annual Core Administrators Workshop - Susan Meyn and Nicole White

Session I: 11am-12pm  (general session, first hour)
    1. S10 RFI Submissions – How should the program be different?
      1. Soliciting feedback on the program
      2. Discuss thoughts and concerns about the S10 program and its direction/approaches
    2. Tracking Shared Facility Resources
      1. Acknowledgements and Citations
      2. Methods employed, informatics approaches  
      3. Alternative suggestions

Session II: 12pm-3pm (Administrative Session, second hour following lunch pickup)
    1. Policies and Implementation Strategies around:
      1. Data Retention Policies
      2. Data Repository Polices and Management of Systems
      3. Biobanking Samples and Centralization efforts
      4. Biobanking policies and Management

Imaging Mass Spectrometry - Michelle Reyzer, Kristie Rose, and Hayes MacDonald

Mass spectrometry is a rapid and sensitive way to obtain molecular information from various samples, and a number of ionization methods are compatible with solid samples such as biological tissues.  Applied in two dimensions, where individual mass spectra are obtained at discrete positions over a sample surface, MS is a powerful imaging technology, capable of determining the distributions of hundreds of individual analytes simultaneously across an entire tissue section.  Imaging Mass Spectrometry has been utilized to detect proteins, peptides, lipids, metabolites, and exogenous compounds from a variety of tissue samples.  This workshop is designed to give the attendees an introduction into experimental considerations when designing an imaging mass spectrometry experiment.  Variables related to sample preparation (including sample accrual, sectioning, and pre-treatment), matrix application (picking the optimal matrix, approaches to matrix deposition), and instrumentation (mass range, mass resolution, spatial resolution) will be discussed with an overall goal of understanding how each one affects the imaging experiment.  Following the workshop, tours will be given at the Mass Spectrometry Research Center at Vanderbilt University where participants will be able to see the imaging workflow and instrumentation in the Tissue Imaging Core facility.

Next Gen Sequencing / Genomics Workshop - Shawn Levy
Generously Sponsored by Illumina 

12:00-12:15 - Introductions, welcome, and agenda

12:15-1:00 - 30x genome performance for 15x genome costs on the HiSeq

1:00-1:30 - Low complexity sequencing and environmental genomics; methods and considerations.

1:30-2:00 - Exceptional libraries from terrible RNA.  RNA quality doesn’t have to be limiting

2:00-2:40 - Novel Illumina applications and synthetic long read technology (Illumina presentation)

2:40-3:00 - Wrap up and open discussion

Correlative Light and Electron Microscopy: Practical Approaches - Chris Gilpin, Josh Rapport, Gregor Heiss
Sponsored by FEI

The aim of the workshop is to cover aspects of correlative light and electron microscopy. FEI Company will talk about their correlative workflow approaches using integrated as well as non-integrated systems. There will also be two presentations from working microscopists who have used correlative light and electron microscopy. After the presentations there will be an open forum discussion session where everyone will be encouraged to ask questions and to provide experiences.